Welcome to BNA company!

No. 1688, Gaoke East Road,Shanghai, China.

Call us anytime

0086-21-58386256

Opeening Time

Mon-Sat: 8.30-18.00

Diffusion blotting for rapid production of multiple ...

Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support. Olsen I(1), Wiker HG. Author information: (1)Department of Animal Health, National Veterinary Institute, 8156, 0033, Oslo, Norway.

signature=0ff97bb456772726110bbbea4fca8d7a ...

Alternatively, a developing buffer containing 5% acetic acid and 10% sodium dodecyl sulfate (SDS) may be used for higher protein extract efficiency. FIG. 2B shows an SEM image of a polished CNT array after PPn coating as in fourth step 253 (inset: the CNT cross-section showing the centered pit after polishing, scale bars are 2 μm and 100 nm, respectively).

Western Blotting: An Introduction - SpringerLink

Olsen I, Wiker HG (1998) Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support. J Immunol Methods 220:77–84 PubMed CrossRef Google Scholar. 45.

Diffusion Blotting for Rapid Production of Multiple ...

Diffusion Blotting for Rapid Production of Multiple Identical Imprints from Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis on a Solid Support Authors: Ingrid Olsen 1, Harald G. Wiker 1 Ingrid Olsen 1, Harald G. Wiker 1 show more details. Series: ...

polyacrylamide in gel electrophoresis producers from ...

Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid. 1. Methods Mol Biol. 2009;536:35-8. doi: 10.1007/978-1-59745-542-8_5.

ACS Applied Bio Materials

Spotlights & Reviews. Articles focused on active research areas, wherein an emphasis is placed on the relationship between the materials or interface synthesis, structure and/or properties and applications.

Diffusion blotting for rapid production of multiple ...

Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) as introduced by Shapiro et al. has become a widely used method for high resolution separation of proteins (Shapiro et al., 1967). In this procedure the proteins react with strongly negatively charged SDS molecules and are separated according to size.

Localizing proteins by tissue printing.

sodium dodecyl sulfate or Tween-20. The following procedure was used to localize potato lectin by means of polyclonal antibodies against the native and deglycosylated potato tuber lectin (5). 1. Cut a section of the tissue about 1 mm thick with a new razor blade, and gently ((. ((.

Neonatal phenobarbital imprints overexpression of ...

Neonatal phenobarbital imprints overexpression of cytochromes P450 with associated increase in tumorigenesis and reduced life span Arun K. Agrawal and Bernard H. Shapiro Laboratories of Biochemistry, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6048

Intracellular growth of Mycobacterium avium ... - BMC …

Olsen I, Wiker HG: Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support. J Immunol Methods. 1998, 220: 77-84. 10.1016/S0022-1759(98)00147-1. …

Simultaneous Immunoblotting Analysis with Activity Gel ...

Olsen I, Wiker HG (1998) Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a solid support. J Immunol Meth 220:77–84 Kurien BT, Scofield RH (1997) Multiple immunoblots after non-electrophoretic bidirectional transfer of a single SDS-PAGE gel with multiple ...

GmKIX8‐1 regulates organ size in soybean and is the ...

Proteins were separated by electrophoresis with 12% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) gel and detected by immunoblotting with a polyclonal anti-GFP antibody (Invitrogen, A - 11122, 1:1000). Ponceau S (Sigma) staining was performed as a loading control. Dual luciferase reporter assays

Model for thermodynamics of ionic surfactant solutions. 2 ...

Size of Sodium Dodecyl Sulfate Micelles in Aqueous Solutions as Studied by Positron Annihilation Lifetime Spectroscopy. The Journal of Physical …

Virus Imprinted Particles - unibas.ch

SDS sodium dodecyl sulfate SDS-PAGE sodium dodecyl sulfate polyacrylamide gel electrophoresis SNPs silica nanoparticles SPR surface plasmon resonance ss single stranded TBSV tomato bushy stunt virus TEOS tetraethylorthosilicate TMPS trimethoxypropylsilane TMV tobacco mosaic virus TNV tobacco necrosis virus

Monodisperse polymer particles with molecular imprints of ...

Methods of seed heterophase polymerization of ethylene glycol dimethacrylate and its copolymerization with styrene or trimethylolpropane trimethacrylate with the aim to prepare monodisperse cross-linked core-shell particles were studied. The possibilities of molecular imprinting for forming sites for molecular recognition of naphthalene molecules were examined.

Sodium Lauryl Sulphate (Inactive Ingredient) - Drugs.com

Sodium lauryl sulfate (C12H25SO4Na) is a surface-active agent used in cleaning and cosmetic products. It is also known by the term sodium dodecyl sulfate. It is used in industrial strength degreasers, floor cleaners, bubble bath and toothpastes. In the pharmaceutical industry, it has been used as an excipient in dissolvable dosage forms.

Altered retinoid metabolism gene expression in chronic ...

Total protein was extracted from the imprints and estimated as published by us earlier.11 ALDH1a1, RARA, RARG and α-tubulin were analysed by western blot. Thirty micrograms of protein was loaded onto 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis and transferred onto a polyvinylidene difluoride membrane (BioRad Laboratories, USA).

Isolation and screening of biopotential endophytic ... - SRFP

For the isolation of DNA, Sodium Dodecyl Sulphate (SDS) method was applied to extract the genomic DNA of endophytic fungi. 500µl of lysis buffer (tris-Cl -50mM, EDTA-100Mm, NaCl-150mM) was taken in Eppendorf tube and o.2gm fresh fungus culture was added in tube and crushed gently. 0.1 vol of 10% SDS was added and incubated at 37ºC for 2 hours ...

Adaptation to inflammatory acidity through neutrophil ...

Acute intestinal inflammation includes the early accumulation of neutrophils (PMN). Based on recent evidence that PMN infiltration "imprints" changes in the local tissue environment through local oxygen depletion and the release of adenine nucleotides, we hypothesized that the interaction between transmigrating PMN and intestinal epithelial cells (IECs) results in …

(PDF) Introduction to Protein Blotting

identical imprints from sodium dodecyl sulfate . polyacrylamide gel electrophoresis on a solid. support . J Immunol Methods 220, 77 – 84 . 45 . Chen, H. and Chang, G.D. (2001) Simulta-

Repression of FGF signaling is responsible for ... - ijbs

Inc.). 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) was used to separate total protein samples and transferred to a PVDF membrane (Millipore) following 5% skim milk blocking for 1 h at room temperature.

(PDF) Western Blotting: An Introduction - ResearchGate

imprints from sodium dodecyl sulfate poly-acrylamide gel electrophoresis on a solid sup-port. J Immunol Methods 220:77–84 . 45. Chen …

A molecular-imprint nanosensor for ultrasensitive ...

Molecular imprinting is a technique for preparing polymer scaffolds that function as synthetic receptors1,2,3. Imprinted polymers that can selectively bind organic compounds have proven useful in ...

Dip-formed synthetic polyisoprene latex articles with ...

The surfactant package includes potassium caprylate, sodium dodecyl benzene sulphonate and polyoxyethylene cetyl/stearyl ether with surfactants in the range of 0.3 to 1.5 wt %; Winsgtay L or butylated reaction product of p-cresol & dicyclopentadiene anti-oxidant preservative is in the range of 0.3 to 1 wt %; ammonium hydroxide is in the range ...

Western Blotting: An Introduction

Proteins that are resolved on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE) gels are typically transferred to adsorbent membrane supports under the influence of an electric current in a procedure that is known as western blotting (WB) or protein blotting (1,2) (see Chapters 2 and 3). Nucleic acids are routinely ...

Neonatal phenobarbital imprints overexpression of ...

Neonatal phenobarbital imprints overexpression of cytochromes P450 with associated increase in tumorigenesis and reduced life span. Arun K. Agrawal, Arun K. Agrawal. ... Briefly, 10 µg of microsomal protein were electrophoresed on 0.75 mm thick sodium dodecyl sulfate polyacrylamide (7.5%) gels and electroblotted onto nitrocellulose filters.

(PDF) Introduction to Protein Blotting

identical imprints from sodium dodecyl sulfate . polyacrylamide gel electrophoresis on a solid. support . J Immunol Methods 220, 77 – 84 . 45 . Chen, H. and …

(ab13524) Anti-LAMP2 antibody [GL2A7] - Abcam - CiteAb

Tissue, cells or virus corresponding to Mouse LAMP2. Purified preparation of mouse liver lysosomal membranes. Protein Names. Lysosome-associated membrane glycoprotein 2, CD107 antigen-like family member B, LGP-96, LAMP2_HUMAN. UniProt Code History. A8K4X5, D3DTF0, P13473, Q16641, Q6Q3G8, Q96J30, Q99534, Q9UD93. Gene Synonyms.

Immunohistochemistry using a Mycobacterium …

Diffusion blotting for rapid production of multiple identical imprints from sodium dodecyl sulfate polyacrylamide gel electrophoresis on a …

Antibody Response to Long‐term and High‐dose ... - Wiley

Sodium dodecyl sulfate – polyacrylamide gel electrophoresis (SDS-PAGE) was performed with the Pharmacia system for horizontal electrophoresis in a Multifor II electrophoresis unit 2117 (Pharmacia LBK Biotechnology AB, Uppsala, Sweden) with precast polyacrylamid gels (ExcelGel SDS gradient 8–18; Amersham Pharmacia Biotech, Uppsala, Sweden ...